Halo-Ed: Molecular Genetics Tutorial (MolGenT)

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About MolGenT

Geneticists have identified certain model organisms which are useful for studying genetic processes in cells. Among the bacteria, E. coli, which is commonly found in the mammalian intestinal tract, has been one of the most widely studied species due to its genetic simplicity and the ease with which it may be grown in the laboratory. Bacillus subtilis, typically found in soil environments, has also been intensively studied because of its tolerance for genetic modification and its ability to produce commercially available biochemicals. Among plants, Arabidopsis thaliana, a mustard weed, has been selected as a model organism due to its high reproductive rate and relatively small genome size. These features make this small weed simpler to genetically manipulate than more complex plant species. Within the Fungi kingdom, the bread mold Neurospora crassa has been chosen as a representative organism because its haploid genome allows the effects of mutations in its DNA to be directly observed. In addition, Neurospora’s short life cycle is useful for efficiently studying patterns of genetic inheritance. Baker’s yeast, or Saccharomyces cerevisiae, is another model organism that has been valuable to genetic research. Yeast is an advantageous organism to study because it is a simple, unicellular eukaryote that is easy to grow and genetically manipulate. Among animals, the fruit fly Drosophila melanogaster is used as a model organism mainly due to its short life cycle and high reproductive rate. Because of these attributes, the inheritance of genetic traits and mutations in this organism can be traced through many generations in a relatively short period of time. Another useful animal model is the mouse. Recently developed techniques allow scientists to breed mice with intentionally engineered mutations in particular genes. By tracking the effect of these mutations, it is possible to identify why certain genes are required and what functions they serve. Finally, the worm C. elegans has been chosen as a developmental model because it is a transparent organism where all cells are visible when viewed under a microscope. This feature is useful because it allows researchers to directly observe changes in the growth patterns of individual cells. Among Archaea and extremophilic bacteria – those which grow in extremely salty or high temperature environments - Halobacteria are good model organisms because they are easy to culture and manipulate in the laboratory. Furthermore, the biochemical machinery of Archaea resembles that of eukaryotes and could therefore be useful in understanding the workings of higher organisms.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

For Questions and Suggestions, contact the Halo-Ed Team

 

Inheritance

Evolutionary Tree

                  Eukaryotic Cells

Cell Cycle

Mitosis

Meiosis

                   Prokaryotic Cells

Binary Fission

Transformation

Conjugation

                     Viruses

                              Bacteriophages

                              Transduction

                             Animal Viruses

                     Model Organisms

DNA & Genes

Nucleotide Structure

Structure of DNA Bases

Base Pairing

Deoxyribose 5' & 3' Ends

Deoxyribonucleotides

DNA Structure

DNA Double Helix

DNA Replication

                    Errors in Replication

DNA Replication, Repair and Recombination

DNA Replication

                    Ladder

                    Helix

Repair of UV Damage

Homologous Recombination

DNA Supercoiling

Gene Expression

          DNA → RNA → Protein

Central Dogma

Genotype vs Phenotype

Phenotype

RNA and Protein Building Blocks

Structure of RNA Bases

Ribose

Ribonucleotides

Amino Acids

Acidic

Basic

Polar

Apolar

Transcription and RNA Processing

RNA Splicing

Translation

tRNA Charging

Genetic Code

Operon

Biotechnology Applications

Impact of Molecular Genetics

Molecular Cloning

PCR Amplification

Protein Expression

DNA Fingerprinting

Genetic Enhancement

Cloning of Animals

CRISPR

Genome Sequencing

Personalized Medicine

Bioremediation

Agriculture and GMOs

          Intellectual Property

MolGenT Test

Copyright © Shiladitya DasSarma